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Chinese Journal of Experimental and Clinical Virology ; (6): 488-491, 2008.
Article in Chinese | WPRIM | ID: wpr-332457

ABSTRACT

<p><b>OBJECTIVE</b>To establish an quick, sensitive and specific assay for effective inactivatian test of inactivated hepatitis A vaccine.</p><p><b>METHODS</b>effective inactivatian test of inactivated hepatitis A vaccine were carried out using integrated cell culture/strand-specific RT-PCR (ICC/strand-specific RT-PCR) assay compared with traditional ELISA and nest RT-PCR assay.</p><p><b>RESULTS</b>all the samples were infectious negative detecting by both ICC/ strand-specific RT-PCR and ELISA assay,while some samples appeared false positive detecting by nest RT-PCR.</p><p><b>CONCLUSION</b>ICC/strand-specific RT-PCR assay is a novel, rapid, sensitive and reliable method for effective inactivatian test of inactivated hepatitis A vaccine. Shorting detection period largely, this assay may be used as an alternative method for routine inactivated hepatitis A vaccines test.</p>


Subject(s)
Animals , Biotechnology , Methods , Cell Culture Techniques , Cells, Cultured , Enzyme-Linked Immunosorbent Assay , Hepatitis A Vaccines , Metabolism , Reverse Transcriptase Polymerase Chain Reaction , Methods , Sensitivity and Specificity , Vaccines, Inactivated , Metabolism , Viral Vaccines , Metabolism , Virus Cultivation , Methods , Virus Inactivation
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